The new policy could be substantial for some Twitter users because follower totals serve as a top selling point when celebrities and so-called social media influencers negotiate deals with advertisers.
They are the two most popular Twitter accounts, with 110 million and 107 million followers respectively before the purge.
Jack Dorsey, Twitter chief executive officer, also lost 200,000 followers.
Trump's account on Wednesday showed him with 53.4 million followers.
Union minister Smriti Irani, who has, of late, been lying low on Twitter, lost close to 40,000 followers (8.19 million) while minister without portfolio Arun Jaitley was down by over 50,000 followers (13 million). While it excludes spam accounts in reported user data, Twitter noted early this year that monthly active user numbers would be "negatively impacted" by efforts to expunge fake accounts.
On Wednesday, Twitter had announced in a statement that it would be deleting the accounts that had been locked, leading to a fall in follower counts this week.
Katy Perry has maintained her place as the most followed person on Twitter.
Former US President Obama lost 2.5 million followers; Trump saw his follower count drop by roughly 400,000 and Kim Kardashian unloaded 1.7 million followers in the last day.
Other high-profile figures also took a hit to their numbers of followers. Obama, for example, gained almost 2,000 followers in the overnight hours, which roughly fits the range of followers he gained in previous weeks during the same Thursday-to-Friday overnight interval.
"People having an artificially-inflated follower count made up of bots and redundant accounts is at best deceiving and at worst, fraud".
Other accounts were locked for tweeting large amounts of unwarranted replies or mentions, misleading links, or "if a large number of accounts block the account after mentioning them". Officials said this will help make Twitter a "more trusted service for public conversation".
Ahead of their highly-publicized Ireland tour, the couple was spotted outside of Westminster Abbey together on Tuesday. Furthermore, she carried an oversized brown Strathberry tote bag and teamed her attire with suede pumps by Givenchy .
Trump has quipped about the USA going it alone before, only to have the administration walk back his comments. Still, Trump has been more conciliatory behind the scenes, including at a leaders' dinner Wednesday.
Photos from the christening of Prince Louis showed that if anyone was nervous before the ceremony in the chapel Royal St. Kate Middleton went with an icy blue dress by her go-to designer, Alexander McQueen, and a hat by Sean Barrett .
Johnson & Johnson says they're " deeply disappointed in the verdict, which was the product of a fundamentally unfair process". Jurors in California previously ordered the company to pay $ 417 million , but J&J successfully appealed that decision.
The man whose auto was stolen found the entire thing hilarious, and gave a healthy "chuckle" when his vehicle was returned to him. Neither of the cars required keys to start the engine instead using key fobs, which the owner left in the vehicle.
Incidents during reports from the World Cup have drawn attention to the journalist's movement to end sexism and harassment. Addiechi added that it was not a "proactive campaign" but said "we'll take action against things that are wrong".
The Mariners had a deal with the chain in which if the team scored five runs, a Papa John's pizza was half-off the next day. Since then, the fallout has been swift: Schnatter resigned from the school's board of trustees and as Papa John's chairman.
Strzok was removed from Mueller's team when the texts were discovered previous year . Earlier he said Page appears to have "something to hide" by not appearing.
The left’s Supreme Court playbook: Zero-tolerance for Dems
The ruling effectively prohibited states from banning abortion before a fetus could be considered viable outside of the womb. Still, they indicate that preventing women from getting abortions is still a top priority for conservatives in the state.
Chelsea sack manager Antonio Conte
His future has looked uncertain with Chelsea failing to qualify for the coming season's Champions League after finishing fifth. Chelsea take on Huddersfield Town in their opening fixture of the new Premier league campaign on August 11.
Illinois Man Calls Woman's Puerto Rican Flag Shirt 'Not American'
In the footage, the man can be seen approaching Irizarry saying: "You should not be wearing that in the United States of America". "Are you educated?" Puerto Rico has been a US territory since 1898 and is classified as a Free Associated State or commonwealth.
PH lauds Thailand for daring cave rescue of boys soccer team
Narongsak, giving details of the rescue, said falling oxygen levels inside the cave complex had added a sense of urgency. Players from France and England welcomed news of the rescue and sent their best wishes to the "Wild Boars" on Twitter .
Trump shares 'very nice note' from Kim Jong Un
An estimated 30,000 U.S. soldiers died in the 1950-53 Korean war . "Midday today they contacted us and offered to meet on Sunday". President and for the improvement of relations between the two countries and the faithful implementation of the joint statement".
Video of high risk rescue in Thai cave
He said, "The situation went beyond just being a rescue mission and became a symbol of unity among man". To get through flooded areas, the children were strapped to a rescue diver and guided through.